Journal: European Journal of Immunology

Article Title: Spatially Resolved Profiling of Compartmentalized Muscle and Brain Inflammation

doi: 10.1002/eji.70119

Figure Lengend Snippet: Conceptual framework for spatially informed tissue profiling. High‐throughput molecular phenotyping via single‐cell or single‐nucleus RNA sequencing enables the characterization of expression in each cell. Cell types or states can be clustered following dimensional reduction, most commonly UMAP. Individual genes or small subsets can be localized in spatial transcriptomic datasets. At the same time, transcriptional identities (like cell types) can be projected onto spatial maps, integrating both modalities. From there, several downstream applications may be used. Including Single gene validations using orthogonal imaging‐based methods like FISH or IHC Functionally validate spatial findings in culture models in vitro or in animal models in vivo Expand the analysis to incorporate other modalities (omics) that in turn may be integrated. Created with Biorender.com.

Article Snippet: High‐resolution spatial transcriptomic atlas of mouse soleus muscle: Unveiling single cell and subcellular heterogeneity in health and denervation , Jer‐En Hsu , 2024 (preprint) , Spatial Transcriptomics ( Seq‐Scope 3–7 dpi) paired with Histology , Mouse (6 months) , Denervation (surgical nerve removal) , Soleus (with and without sciatic nerve) , Novel hybrid fibers at a transitional state Mapped NMJ nuclei Fiber differences to stress and Denervation‐induced ECM remodeling dynamics.

Techniques: High Throughput Screening Assay, RNA Sequencing, Expressing, Imaging, In Vitro, In Vivo

Journal: Journal of Toxicologic Pathology

Article Title: Intramuscular collagen accumulation in different types of skeletal muscle fibers in middle-aged male rats

doi: 10.1293/tox.2025-0072

Figure Lengend Snippet: Hematoxylin and eosin staining of rat skeletal muscles. Left and right images represent the extensor digitorum longus (EDL) and soleus (SOL) muscles, respectively. Panels A and B show the young group, whereas panels C and D show the middle-aged group. Scale bars, 50 μm.

Article Snippet: The extensor digitorum longus (EDL) and soleus (SOL) muscles from male Sprague–Dawley (SD) rats were collected and sectioned.

Techniques: Staining, Muscles

Journal: Journal of Toxicologic Pathology

Article Title: Intramuscular collagen accumulation in different types of skeletal muscle fibers in middle-aged male rats

doi: 10.1293/tox.2025-0072

Figure Lengend Snippet: (A–D) Representative Picrosirius Red staining images. Left and right images represent the extensor digitorum longus (EDL) and soleus (SOL) muscles, respectively. Panels A and B show the young group, whereas panels C and D show the middle-aged group. Scale bars, 50 μm. (E) Quantification of Picrosirius Red-positive collagen fiber content (% area) in the EDL and SOL of each age group. Data are presented as mean ± standard error of the mean (n=6 per group). *p<0.01 between groups (one-way analysis of variance with Bonferroni post hoc test).

Article Snippet: The extensor digitorum longus (EDL) and soleus (SOL) muscles from male Sprague–Dawley (SD) rats were collected and sectioned.

Techniques: Staining, Muscles

Journal: Journal of Toxicologic Pathology

Article Title: Intramuscular collagen accumulation in different types of skeletal muscle fibers in middle-aged male rats

doi: 10.1293/tox.2025-0072

Figure Lengend Snippet: (A–D) Representative hematoxylin staining images. Left and right images represent the extensor digitorum longus (EDL) and soleus (SOL) muscles, respectively. Panels A and B show the young group, whereas panels C and D show the middle-aged group. Scale bars, 50 μm. (E) Analysis of the number of nuclei per unit area (mm 2 ) in the EDL and SOL of each age group. Data are presented as mean ± standard error of the mean (n=6 per group). *p<0.01 between groups (one-way analysis of variance with Bonferroni post hoc test).

Article Snippet: The extensor digitorum longus (EDL) and soleus (SOL) muscles from male Sprague–Dawley (SD) rats were collected and sectioned.

Techniques: Staining, Muscles

Journal: Journal of Toxicologic Pathology

Article Title: Intramuscular collagen accumulation in different types of skeletal muscle fibers in middle-aged male rats

doi: 10.1293/tox.2025-0072

Figure Lengend Snippet: Spearman’s rank correlation coefficients showing the relationship between collagen fiber content (Sirius red % area) and the number of nuclei per unit area (mm 2 ) in the extensor digitorum longus (EDL) and soleus (SOL) muscles of all animals (n=6 matched data points for each age group and each muscle). Open and black circles represent the EDL of the young and middle-aged groups, respectively. Open and black triangles represent the SOL of the young and middle-aged groups, respectively.

Article Snippet: The extensor digitorum longus (EDL) and soleus (SOL) muscles from male Sprague–Dawley (SD) rats were collected and sectioned.

Techniques: Muscles